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DAx Data Acquisition and Data Analysis

What users say


Asked about the advantages of DAx over other software packages for DNA trace analysis, David O'Connor, Ph.D, Assistant Professor, Department of Pathology and Laboratory Medicine, Wisconsin National Primate Research Center, University of Wisconsin-Madison (e-mail address available on request) writes:
The big advantages of DAx are flexibility in the types of data that can be analyzed, flexibility in workflow design, technical and customer support, and price.
Also, DAx runs fine on Macintosh OSX under Parallels and VMWare Fusion running XP or Vista virtual machines.

Shelby O'Connor, Ph.D, Assistant Scientist, Department of Pathology and Laboratory Medicine, Wisconsin National Primate Research Center, University of Wisconsin-Madison (e-mail address available on request) writes:
The allele match sheet tool in DAx has made genotyping MHC alleles far easier than any other tool we've tried! The ability of DAx to recognize multiple file types and then compare them to self-selected reference alleles provides us with an exceptionally powerful tool to genotype our animals.

Alexander Miron, Ph.D, Dana Farber Cancer Institute and Harvard Medical School (e-mail address available on request) writes:
For the daily analysis of several million base pairs of genomic DNA based on heteroduplex analysis DAx has quickly become an indispensable tool.

Lynn S. Penn (1-859-257-7897, email address available on request), Head of the Department of Chemistry at Drexel University, and past President of the Adhesion Society writes:
We conduct lengthy heterogeneous phase reactions in which polymer chains are tethered from dilute solution to the surfaces of solid substrates. To monitor these reactions quantitatively, we use high pressure liquid chromatography to analyze the changing size of the reactant peaks with respect to those of nonreactive internal standards. A vast amount of raw data in the form of chromatograms is collected over the course of a reaction, with data emerging simultaneously from both ultraviolet and refractive index detectors. Only the DAx software (we have tried several others) allows us to process, smoothe, filter, normalize, and analyze these data rapidly and reliably enough to make on-the-spot adjustments to our reacting systems. Our work also requires frequent evaluation of column performance and calculation of the number of theoretical plates. With the DAx software, this is fast and easy. In sum, the DAx software is versatile, flexible, and user-friendly, and we would not think of doing our research without it.

Dr. Stephen Williams PhD, Associate Director of Instruments and Software at ACLARA BioSciences writes:
ACLARA BioSciences has worked with Van Mierlo Consultancy to modify the DAx program to analyze electropherogram trace files from our eTagTM reporter assays. We are very impressed with the flexibility of the DAx program, which has a host of features to allow the user to customize their analysis procedures. We also found the use of the identification database very helpful in identifying our eTag reporter molecules. When it was time to create an eTag assay-specific software program, we built on the tried and tested DAx peak detection algorithms, added some of our own features (with Peter van Mierlo’s programming help), and created an eTag-specific application called eTag InformerTM Software. The software is designed to identify and quantify our eTag reporters from data generated in a 96-well microtiter plate format. We’ve installed the software at several pharmaceutical companies who are currently running eTag reporter assays for genomics applications. We’ve been extremely pleased with the performance of the software.

Torleif Björnson, Sr. Director of Engineering at ACLARA BioSciences writes:
In developing new systems and applications for our micro-fluidic devices, we develop many breadboard systems for use by our scientists. We have found DAx to be a convenient and efficient tool to enable our scientists to analyze the electropherograms generated by such early breadboard systems.
Van Mierlo Software Consultancy has been able to quickly and cost-effectively provide us customized versions of DAx to meet the specific needs of our application.

Dr. R. Hoffmann of Biofrontera Pharmaceuticals, Germany, writes:
We are using DAx currently to analyse DNA-fragments which were run on a MegaBACE 1000 capillary system. DAx offers a lot of features to fully automise the data analysis process, by simply indicating which kind of parameters will be used for the analysis of a DNA size standard trace and which different ones for the analysis of the sample traces. The analysis parameters can be easily adapted to any kind of DNA size standard used.
In addition, the amount of possibilities to change the analysis parameters like raw data filtering, baseline setting, peak detection, allows the user to define the parameters for virtually all kinds of generated data. All predefined analysis functions can be saved in a separate file which is then used for the automatic analysis of thousands of data points.
Overall we find DAx is a very powerful tool for the analysis of DNA-fragments, separated by gel electrophoresis. We particularly like the high grade of automating the data analysis process which is a must for all users who are working with high-throughput data generating systems like the MegaBACE 1000 machine.

Niu Hongsen of Merial Limited, Iselin, NJ, USA writes:
I use DAx for GPC molecular weight and polydispersity measurements. It saved me a lot time in data analysis. It is simple and easy to learn. Nowadays, most large laboratories use network data systems that normally do not have GPC function or CZE feature. DAx will be a very useful tool as an addition data analysis. Because the price advantage and the versatility, it can be really valuable to small companies and academic research laboratories.
Another thing I like is you can always talk to DAx people and get a very quick response for problems.

A user at a large industrial organic chemistry laboratory in the Netherlands writes:
We gebruiken DAx voor data registratie afkomstig van UV detectoren van 2 analytische Waters HPLC-sytemen en 1 preparatief Waters HPLC-syteem. Vanwege plaatsing op 3 verschillende laboratoria staan de systemen nu op aparte PC's aangesloten. In het verleden hebben we echter op 2 van deze PC's dmv DAx zonder problemen minimaal 2 detector-signalen tegelijkertijd geregistreerd. Laatstgenoemde opzet bespaart uiteraard op kostbare laboratoriumruimte en PC's.

De gebruikers van deze HPLC-systemen ervaren DAx als gebruiksvriendelijk en robuust. Er zijn weinig storingen, en als er storingen optreden dan ligt de oorzaak vrijwel nooit in DAx, maar is deze bijna altijd terug te voeren naar enerzijds de aangesloten elektronica en anderzijds de instabiliteit van het operating system. Sinds we enkele maanden geleden van Windows 3.11 zijn overgegaan op Windows 95, draait DAx uiterst stabiel en zijn er ook geen storingen meer gerapporteerd.

Translation: We use DAx to register data coming from UV detectors in 2 analytical Waters HPLC systems and 1 synthesising Waters HPLC system. Since these systems are now placed in separate labs, they have been hooked up to separate PCs. In the past, however, we used 2 of these PCs to acquire at least 2 signals simultaneously using DAx. Such a setup naturally saves valuable laboratory space as well as PCs.

In the experience of the users of these HPLC systems DAx is user friendly and robust. There are few problems, and when problems occur DAx is rarely the cause - almost invariably the electronics or the operating system are at fault. Ever since we upgraded from Windows 3.1 to Windows 95 some months back, DAx has been very stable and no problems have been reported.

Dr. Jetse Reijenga of the University of Technology in Eindhoven writes:
"As an educator, it is important that I can tell my students how to use the program to do what they need to do FAST. With DAx it takes me just five minutes."

Joost van Dongen of the University of Technology in Eindhoven writes:
In using DAx I have found three great advantages:
  1. DAx can be customised, so that inexperienced users do not get confused by menu options that they do not need. The customisation also serves as a form of security, because it is possible to keep certain parts of the program hidden from unauthorised students.
  2. Measurements can be dragged and dropped. This is very useful to compare measurements. DAx has very good overlay options. It is even possible to overlay measurements that are still running.
  3. Personally I use DAx for SEC (size exclusion chromatography) and HPLC. The SEC calibrations in DAx are very easy to use.

Joop Waterval, PhD of the Department of Pharmaceutical Analysis, University of Utrecht writes:
The program offers a lot of features for the high demanding user, but it can also be operated in a easy-to-use manner by low-experienced persons. It is very flexible program, e.g., quantitation of peaks can be done during the run and results can be presented in about every desired way.

Marco Molling, Prince Technologies We use DAx for capillary electrophoresis. I am quite pleased with the fact that in DAx it is possible to recognise (identify) peaks based upon the peak start or peak end time rather than on the peak top time. Especially when analysing highly mobile ions at various concentrations the peak start time remains constant whereas peak top time may vary as a function of the analyte's concentration. As opposed to most other programs this is not a problem in DAx.

Berton van Asseldonk, Prince Technologies Despite the many features and options in DAx, the design of the program is very logical, so it was easy to learn how to use DAx.