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DAx has all the features that are needed for chromatographic applications in
general, such as the ability to measure sequences of measurements, recognise and
quantify components, and compare measurements.
It also contains a number of features geared specifically towards Size Exclusion Chromatography / Gel Permeation Chromatography:
New application note:
This application note also serves as a manual on the interpretation of
molecular weight distribution data in text files.
 | Calibration Curves relating Molecular Weight to Elution Volume |
Calibration curves can be multi-linear, cubic spline, or polynomial (up to 10th order).
Curves can be plotted linearly or logarithmically.
Peak lists can display one or all of
- Mp (mol weight at peak top)
- Mn (number averaged mol weight)
- Mw (weight averaged mol weight)
- Mz
- D (dispersivity)
- Mol weight at peak start / Mol weight at peak end
| Mw Axes |
Traditionally, chromatographic measurements have a horizontal axis depicting
time coordinates. Using an Mw calibration, DAx can convert measurements
to have a horizontal axis depicting molecular weight or log(Mw).
| Converting between concentration and weight data |
Chromatographic measurements typically measure component concentration or
component weight. The distinction is important when calculating peak parameters
and when drawing molecular weight distributions.
| Identifying components by molecular weight |
Components can be identified by Mp, Mn, Mw or Mz.
| Molecular weight axes |
Data can be plotted with a molecular weight axis instead of a time axis. If baselines
are present, they can be automatically subtracted from the data to give a much cleaner plot.
The plots can be normalised so that the accumulative molecular weight in all peaks that
are present is 100%, making it very easy to compare molecular weight distributions
between different measurements.
| Mouse tracking |
When the mouse cursor is tracked over a graph, the molecular weight at the cursor's coordinate
is displayed.
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